Western blot is a kind of hybrid technique which combines high-resolution gel electrophoresis and immunochemical analysis techniques. Western blot, constructed with several advantages including high volume, high sensitivity and high specificity of testing, etc, is a widely used method for detecting the properties, distribution of proteins, including but not limited to the qualitative and quantitative detection of tissue antigens that determine quality. polypeptide molecules and antibodies or antigens used to detect viruses. You can visit some sites like http://bosterbio.com to get information on blot technology.
Labour theory
The mixed antigen samples were separated by unidirectional or bidirectional electrophoresis on the gel plate and then an immobilized matrix membrane was applied to the gel. The individual antigen components in the gel are transferred to the printing paper and solid phase by means of natural adsorption forces, electric field forces or other external forces. And then, immovable matrix membranes are detected and analyzed by immuno-coating solution techniques such as immunoisotope probes or immune enzyme probes.
Benefits
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Western blot is a technique used to analyze antigens and antibodies. This has the following advantages:
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Wet immobilization matrix film is flexible and easy to use;
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Immovable biomolecules can be in close proximity to different immune probes and are not blocked as a gel by pore size.
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Western blot analysis requires very little reagent.
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Incubation and washing times are significantly reduced;
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Multiple copies can be made simultaneously for multiple analysis and identification.